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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
05/12/2018 |
Data da última atualização: |
09/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FIGUEIREDO, A.; AGNOLON, I. C.; LOPES, L. G.; GIGLIOTI, R.; CHAGAS, A. C. de S. |
Afiliação: |
Amanda Figueiredo, UNESP; Isabela Cabeça Agnolon, UNICEP; Louyse Gabrielli Lopes, UNICEP; Rodrigo Giglioti, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Comparative study of hatching estimation methods of Rhipicephalus (Boophilus) microplus eggs. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Veterinary Parasitology, v.264, p.35-38, dec. 2018. |
ISSN: |
1873-2550 |
Idioma: |
Inglês |
Conteúdo: |
Reproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to improvement of the AIT and can stimulate researchers to choose more accurate techniques for the assessment of egg hatching. MenosReproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to impro... Mostrar Tudo |
Palavras-Chave: |
Adult immersion test (AIT); Cattle tick; Methods of larvae estimation; Quantification by sampling; Rhipicephalus (Boophilus) microplus; Visual estimation. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187834/1/ComparativeStudy.pdf
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Marc: |
LEADER 02408naa a2200253 a 4500 001 2100813 005 2019-01-09 008 2018 bl uuuu u00u1 u #d 022 $a1873-2550 100 1 $aFIGUEIREDO, A. 245 $aComparative study of hatching estimation methods of Rhipicephalus (Boophilus) microplus eggs.$h[electronic resource] 260 $c2018 520 $aReproductive parameters of Rhipicephalus (Boophilus) microplus are often evaluated. They are good indicators of resistance to commercial acaricides and of plant extracts? efficacy. The objective of this study was to compare the techniques: visual estimation and quantification by sampling used in the Adult Immersion Test (AIT) to calculate the hatching rate of eggs. Engorged females collected from cattle were subjected to the AIT with plant extracts and kept in an incubator for oviposition. The egg hatching was evaluated in 210 syringes by visual estimation (%). Then, eggs and larvae were counted into samples of 100 individuals, in three repetitions by stereo microscope. Significant differences were found between the two tests (p?0.05). The egg hatching average of visual estimation was higher than the quantification by sampling, 56.8?48.0, respectively (correlation=0.85). We found that the visual assessment leads to a higher estimate of larvae in relation to eggs, because the infertile eggs can be concealed in the center of the syringe. In quantification by sampling, no statistical differences (p=0.99) were observed in the pairwise counts between the three samples (48.1 ± 26.6%, 47.8 ± 26.9%, 48.1 ± 26.5%) (correlation of repetitions=0.96). This suggests that counting one sample is sufficient and the result should not differ much, regardless of the evaluator. Regarding the cutoff point of tick resistance status (95%), both methods are reliable. This study contributes to improvement of the AIT and can stimulate researchers to choose more accurate techniques for the assessment of egg hatching. 653 $aAdult immersion test (AIT) 653 $aCattle tick 653 $aMethods of larvae estimation 653 $aQuantification by sampling 653 $aRhipicephalus (Boophilus) microplus 653 $aVisual estimation 700 1 $aAGNOLON, I. C. 700 1 $aLOPES, L. G. 700 1 $aGIGLIOTI, R. 700 1 $aCHAGAS, A. C. de S. 773 $tVeterinary Parasitology$gv.264, p.35-38, dec. 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
10/01/2008 |
Data da última atualização: |
12/02/2009 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Circulação/Nível: |
-- - -- |
Autoria: |
MELO, R. O. de; PACHECO, E. P.; CUNHA FILHO, M.; CANTALICE, J. R. B. |
Afiliação: |
Rogério Oliveira de Melo, Universidade Federal Rural de Pernambuco; Edson Patto Pacheco, Embrapa Tabuleiros Costeiros, Moacyr Cunha Filho, Universidade Federal Rural de Pernambuco; José Ramon Barros Cantalice, Universidade Federal Rural de Pernambuco. |
Título: |
Susceptibilidade à compactação e correlações entre propriedades físicas de um neossolo sob vegetação de caatinga. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira: anais. Gramado: SBCS, 2007. 1 CD ROM. |
Idioma: |
Português |
Palavras-Chave: |
Propriedade física. |
Thesagro: |
Caatinga; Solo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00667naa a2200181 a 4500 001 1372131 005 2009-02-12 008 2007 bl uuuu u00u1 u #d 100 1 $aMELO, R. O. de 245 $aSusceptibilidade à compactação e correlações entre propriedades físicas de um neossolo sob vegetação de caatinga. 260 $c2007 650 $aCaatinga 650 $aSolo 653 $aPropriedade física 700 1 $aPACHECO, E. P. 700 1 $aCUNHA FILHO, M. 700 1 $aCANTALICE, J. R. B. 773 $tIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira: anais. Gramado: SBCS, 2007. 1 CD ROM.
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